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KMID : 0380020060210050331
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.331 ~ p.335
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Production of Immunostimulating Polysaccharide in Angelica gigas Nakai Suspension Cell Cultures
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Kim Young-Hwa
Kim Ik-Hwan
Kim Dong-Il
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Abstract
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Suspension cells of Angelica gigas Nakai were cultivated to produce extracellular polysaccharide (ECP) as immunostimulating agents. Effects of environmental conditions such as sucrose and 2,4-dichlorophenoxyacetic acid (2,4-D) concentrations on the growth and production of ECP were studied using suspension cultures of A. gigas Nakai. Final dry cell weight was increased with an increase of initial sucrose concentration from 30 to 60 g/L. The maximum production of ECP (1.2 g/L) was achieved at an initial sucrose concentration of 50 g/L on day 8. High 2,4-D concentration was effective for ECP production but not for cell growth. In addition, various fungal elicitors were investigated for the enhanced production of ECP in A. gigas suspension cultures. Among the tested fungal elicitors, Verticillium dahliae was the most effective for the production of ECP in A. gigas suspension culture.
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KEYWORD
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Angelica gigas, extracellular polysaccharide, elicitor, plant cells
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